For anyone who wants to learn & touch bases on the DNA tissue culture questions DNA feel free to ask me. I have a clean room area in my lab where marijuana plants DNA cultures I have made and have done to be made and modified and maintained here in my lab, and yes I can store and create thousands of many crazy and unique beautiful marijuana mutant plants with a few DNA chromosome cells and use masses from the DNA tissue cultures, I’m looking to bank the DNA tissue cultures to have hundreds of strains available without any seeds (or make artificial seed with DNA tissue cultures in the flask seed crisper tube), at any of a moments notice can create them. The good part is you test the plant once and it should always be the same mutated marijuana plant DNA and the same CBD/THC levels, each and every time just as a clone or using a grow tray with marijuana plant tissue DNA cultures but without the cells being mature and the plants life cycle starts over every time you culture it always making it a 1/1 copy every time.
I will be making some polyploid plants by modifying marijuana plants DNA tissue genetics, by subjecting seeds to Colchicine derived from Autumn Crocus (a common flower which is poisonous in nature but the poisons DNA can be genetically removed) by crushing their seeds or extracting from the plants. This has been a big project for me but I think the time is worth the rewards in the end!.
This has been used in the industry to create large flowers in horticulture labs world wide and used in breeding forever without any issues of having male plants appear including hermaphrodites as well. Colchicine is a hormone and is used for gout treatment and over ingesting it can actually kill you but I have managed to removing this from the marijuana's cultures of DNA but if I didn't you don’t want to be smoking the 1st generation of plants, just the ones you grow from the seed you create are by far safest. It mutates the plants to double up the marijuana's plants genetics of it's DNA chromosomes and then it becomes a mutant polyploid, not a diploid like everyone else is growing these days. I have read back on my notes that I had written down and recorded that tetraploids 4n grow slowly. So my ideas was to create a tetraploid, and then pollinate with a standard diploid 2n, which will make it a 3n DNA genetic off spring (pretty much the equivalent of a mule, cannot create offspring sort of) (for reference: A horse has 64 chromosomes, and a donkey has 62. The mule ends up with 63. Mules can be either have male or female, but, because of the odd number of DNA chromosomes in my marijuana's plants DNA genetics, they can’t reproduce and this has been a very long journey for me over the years of DNA chromosomes and marijuana plants DNA and genetics and tissue cultures research that I have done)
Polyploid is a natural mutation in nature by itself in which a cells get extra sets of DNA chromosomes (think of giant redwood trees 66 DNA chromosomes total in DNA) I'm wondering if any of those extra DNA chromosomes make them huge? or do other kinds of unique mutations).
Marijuana plants are usually only diploid 2 DNA chromosomes, which means that they have two complete sets of DNA chromosomes. Those being Polyploids have a higher number of DNA chromosomes sets, so there can be triploid (3n), tetraploid (4n ) on and on. You can keep going higher in this area when it comes to marijuana plants genetics and DNA. But here comes the interesting part that I have been doing.
If you have a triploid (three sets; 3x), for example like seedless watermelons. You can have an outdoor grow that cannot be pollinated ever by regular 2n diploid pollen. or pollen from hemp naturally growing on a hemp farmers fields. So you could breed in a grow room and not accidentally pollinate the plants, sitting next to them. This below here is not my photos, but the general idea is the same none the less, this I do know and believe is the slower growing polyploid.
A common flower that now has doubled up DNA chromosomes and genetics. They look like mutant aliens and freaks of nature,
I know.
However,
I believe if you were to look at some strains that are higher THC they possibly could be triploid 3n plants now. which would explain if you are having issues breeding other strains to it, and the ever increasing CBD, CVB & THC levels.
https://en.wikipedia.org/wiki/Polyploid
I will publish videos on this also whenever I have the time to in the future on here. I have some research on this subject along with the DNA tissue cultures for a number of years now and just started growing this strains that I have been working on and developing for well over 22 years ago. So I will be posting lots of info online here for my own personal record research and for you to learn from as well.
Love to hear anyone try taking on this on there own. I know I have some ambitious projects always ahead of me, but there are hundreds of haters that will say 12/12 from seed is crap, and I bet with some strains sure. But when I find that strain I can produce a lot and have constant colors of my own choosing and genetics of marijuana plants and flowers coming out especially if the marijuana plant is pink and it's buds have a earthy cotton candy sweet teste to them, like my Hawaiian Indica hybrid mutant strain i’ll be happy.
I will even tell you exactly how I managed to create a new white pinkish Hawaiian Indica hybrid mutant strain by genetically making a white mutant ******* marijuana plants DNA that lacks green chlorophyll and combining it's DNA chromosome cultures with a Hawaiian Indica Panama Red marijuana plant giving me a pink marijuana plant!.
